Cell Therapy Engineering and Genome Editing

How Our Technology Improves Cell Therapy Engineering and Genome Editing

Stem cells and T-cells can be engineered to differentiate and form specific cell types or phenotypes respectively. But the identification of the factors that cause reproducible differentiation, and the analysis of the newly generated cells themselves, can be challenging.

At Sphere Fluidics, we’re dedicated to making these challenges simple. Our unique platform enables you to precision engineer, screen and isolate individual cells using automated, miniaturisated and integrated techniques that are significantly faster and more cost-effective than alternatives.

Single cell research

Our Unique Benefits for Cell Therapy Engineering and Genome Editing

Picodroplets provide a unique way of compartmentalising single cells for individual engineering, screening and detailed analysis. Once compartmentalised, these picodroplets can be fused with different factors (e.g. genome editing complexes, single viruses, growth factors, cDNAs, 3D substrata, or engineered micro-gels) to stimulate or transform the cells, which can then be sorted and assayed to isolate specific phenotypes.

Since you can compartmentalise cells using our picodroplet technology, each one can then be engineered on a single cell basis. This removes the biases and inefficiencies seen with the transfection of bulk populations of cells. Our technology also has significant potential for use in the clinic, for example, in the precision reprogramming of T-cells collected specifically from a patient to create personalised medicines. Potentially, we can also minimise the risk of cell damage or oncogenicity by controlling the exact number of genome editing complexes, cDNAs or viruses we introduce into each cell – even to the single molecule level.

Our Technology in Action for Cell Therapy Engineering and Genome Editing

Our picodroplet technology can be used for numerous single cell research applications, including:

  • Controlling the transfection and selection of transformed T-cells for cell therapy
  • Automation and miniaturisation of genome editing
  • Separation of specific phenotypes
  • Investigating the impact of cell-cell or cell-molecule interactions on cellular phenotype
  • Identifying cell instability and variation
  • Studying the differentiation process of single cells

As can be seen in the diagram, our systems provide a simple, fast and high-throughput process. For example, with T-cells, once blood is collected from a patient, target cells are isolated and purified. These cells are then encapsulated into individual picodroplets, which are fused with another picodroplet containing the factor of interest (e.g. a viral construct) and electroporated. Following incubation and sorting, the positive clones or ‘hits’ can then be analysed by DNA/RNA sequencing, proteomic analysis or other phenotyping tools.

In the application of transfection and selection of transformed T-cells for cell therapy, the automation and high precision of our systems will improve cell separation and the overall transduction process. Since this approach compartmentalises cells, they can be collected from a patient, separated into single picodroplets and reprogrammed. This should have a high efficiency meaning that less sample is needed from the patient and generation of a more effective precision therapy.

To find out how you could utilise our technology for cell engineering and genome editing in your lab, get in touch.

Single cell analysis