Why Choose Cyto-Mine®?
There are a number of different techniques that are routinely used in the biopharmaceutical discovery and development workflow, including single cell analysis, sorting, imaging and dispensing into individual wells of microtitre plates. Traditionally, different items of equipment would be required for each technique, resulting in a costly and time-consuming process that uses up valuable lab space and increases risk of sample contamination.
Our Cyto-Mine® technology is the first integrated device to be able to automatically perform all of these crucial techniques in a single compact system. This high-throughput instrument uses picodroplet technology and microfluidics to process around 1 million heterogeneous mammalian cells in less than half a day. Each cell is encapsulated in a picodroplet containing growth media, which acts as a bioreactor to compartmentalise the cell and let it grow; eventually trapping secreted molecules such as antibodies. The unique workflow enables selective screening of single cells to find rare lead candidates.
Since the single cells are compartmentalised, monoclonality is ensured and you can perform novel assays to determine protein secretion rates, titre and antigen-specificity. The disposable Cyto-Cartridge™ used with Cyto-Mine® gives you confidence of sterility and minimises crossover risks. Automation of the system reduces human resource requirements and the simple ‘load-and-go’ format makes it easy to use by everyone in the lab.
There are four main application modes for the Cyto-Mine® system:
- Monoclonality assurance mode – reliably sort single cells into individual wells of microtitre plates.
- Direct assay mode – sort rare clones at high throughput and ensure the most relevant clones are flagged for downstream analysis.
- Stability test mode – identify unstable clones early so that they can be eliminated from the analysis or inform you of “genetic drift” in a cell population.
- Fusion assay mode – sort rare cell-cell or cell-biomolecule partners. This could be useful for constructing a range of functional assays. For example, B-cells could be co-incubate with a target cell to which specific secreted antibodies may bind and cause signal transduction resulting in generation of a fluorescent readout
Why Choose Our Technology?
Because we have a competitive advantage. Our technology is not only more cost-effective, both in terms of initial outlay and ongoing costs, but it delivers quality results.